Rapid communication: a restriction fragment length polymorphism in the porcine leptin receptor (LEPR) gene.
نویسندگان
چکیده
منابع مشابه
Rapid communication: a restriction fragment length polymorphism in the porcine leptin receptor (LEPR) gene.
Polymorphism. A Hinf I PCR-RFLP was identified in the porcine leptin receptor ( LEPR) gene. Source and Description of Primers. Human cDNA (GenBank accession no. U43168) sequence was used to design primers to amplify porcine genomic DNA. Primer Sequences. Forward primer: 5¢GCATCCCATATCTGAACCC3¢; reverse primer: 5¢-CCACTTAAACCATAGCGAATC3¢.
متن کاملRapid communication: SacI restriction fragment length polymorphism in a porcine vascular cellular adhesion molecule (VCAM1) gene.
Probe Source and Description. A randomly selected 1.3-kb cDNA clone (S28) was isolated from a porcine spleen library (CLONTECH j. Amplification and hybridization analysis (previously described; Tuggle and Schmitz, 1994) indicated S28 was specifically expressed in spleen. A 504-bp BgZII-BarnHI subclone of S28 was sequenced and identified as a porcine vascular cellular adhesion molecule ( VCAM) c...
متن کاملRapid communication: StuI restriction fragment length polymorphism at the porcine bone morphogenetic protein 5 (BMP5) locus.
Source and Description of Probe. A 2.2-kb human cDNA clone for bone morphogenetic protein 5 (BMP5) was excised from the clone U2-16 (Celeste et al., 1990).
متن کاملRapid communication: TaqI restriction fragment length polymorphism at the porcine bone morphogenetic protein 6 (BMP6) locus.
Source and Description of Probe. A 1.1-kb human cDNA clone for bone morphogenetic protein 6 (BMP6) was excised from the clone U2-7 (Celeste et al., 1990). Method of Detection. Genomic DNA was isolated from whole blood and digested with TuqI. Fragments were separated by agarose gel electrophoresis and akaline vacuum-transferred to charged nylon membranes. Hybridizations were at 65°C for 16 to 20...
متن کاملRapid communication: restriction fragment length polymorphism at the porcine superoxide dismutase locus.
PoZymorphism. An RFLP was detected in the superoxide dismutase 2 (SOD2) locus of swine using the restriction enzyme StuI. Source and Description of Probe. An 827-bp human cDNA clone for SOD2 was excised from the EcoRI site of plasmid phMnSOD4 (Xiang et al., 1987). Method of Detection. DNA was isolated from blood, digested with StuI, separated by agarose gel electrophoresis, and transferred ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Animal Science
سال: 1997
ISSN: 0021-8812
DOI: 10.2527/1997.7582287x